Store at room temperature. In addition, two highly sensitive spectrophotometric assays have been 상세설명. 10. Xylene Cyanol FF is a tracking dye commonly used for nucleic acids. Fully misicible in water. SDS. 025 g bromophenol blue 0. The product can be stored for up to 12 months. Solubility. Current Protocols in Nucleic 二甲苯蓝(xylene cyanol)又称二甲苯青，可以被用于作为琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳的标记物，相似的，溴酚蓝和橙黄G也可以用于该用途。 迁移速度. Xylene Cyanol FF - Methyl Orange. 6. 5% xylene cyanole. We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production. Add 7 ml deionized / Milli-Q water. Some documentation and label information may refer to the legacy brand. 133 or European Standard EN166. Add 10X native agarose gel loading buffer (15% ficoll, 0. Xylene cyanol FF migrates comparatively slowly and corresponds to the migration of 4000 – 5000 bp long DNA fragments in 1% agarose gel. The three xylene isomers: o -xylene, m -xylene, and p -xylene. It typically takes ~10–13 h for ssDNA (depending on the length) to migrate ~3/4 of the entire gel length. % Acrylamide Effective Range of Separation (bp) Xylene Cyanol (nucleotides) Bromophenol Blue (nucleotides) 3. The migration of the xylene cyanol FF and bromophenol blue dyes along the gel gives an estimate of how far the DNA has migrated (see Note 7). Please Percent Bromophenol Blue (BPB) Xylene cyanol FF (XC) 4% 30 nt 155 nt 6% 25 nt 110 nt 8% 20 nt 75 nt 10% 10 nt 55 nt • For RNase protection (30-50 nt): run 12% gel and stop when BPB is 1-2 cm from bottom. Product Overview. PublicationThis Product Has Been Jun 15, 2024 · Room Temperature (Recommended in a cool and dark place, <15°C) CAS RN. Component CAS No IARC NTP ACGIH OSHA Mexico 1,3-Benzenedisulfonic acid, 4-[[4-(ethylamino)-3-m ethylphenyl][4-(ethylimi . Transfer it to a 15-mL screw-capped graduated tube. 6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. In each case, the formamide tracking dye solution containing bromophenol blue and xylene cyanol FF can be loaded into an unused well to monitor the extent of migration during DNA gel-loading dye (10X) 3. 0, 91% formamide; stored at −20°. A 10X solution of 40% Sucrose, 0. XYLENE CYANOL FF EXTRA PURE Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Catalog number 440700100 . Not for use in diagnostic procedures. CAS No. Reconstituted solution contains 0. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Th Xylene cyanol FF Hãng sản xuất: BioBasic-Canada Tên khác: Acid blue 147, Cyanol FF, XC CAT. It runs alongside different DNA length markers depending on the % of the agarose gel. Telangusadhushetty N. Store at -20°C. In 1% agarose gels, Xylene cyanol typically migrates at about the same rate as a 4000 base pair DNA fragment. Sigma-Aldrich offers precast 1. 5. Stop electrophoresis when the xylene cyanol dye reaches the bottom of the gel. They are derived from the substitution of two hydrogen atoms with methyl groups in a benzene ring Redirecting to /NL/en/sds/SIGMA/X4126?userType=undefined. This chemical belongs to the triarylmethane dye family and is recognized for its distinct blue-green color. Product Name Xylene cyanol FF Cat No. Store at Room Temperature. C 25 H 27 N 2 NaO 6 S 2. Dilute 1:10 for nucleic acid electrophoresis in agarose or polyacrylamide gels or add 4 parts formamide to 1 part dye solution for DNA sequencing. A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0. On native gels, the samples can be loaded directly without heating. : BP125-100 CAS No 4463-44-9 Synonyms C. • For 5. Bromophenol blue and orange G can also be used for this purpose. Details of the supplier of the safety data sheet Emergency Telephone Number CHEMTRECÒ, Inside the USA: 800-424-9300 CHEMTRECÒ, Outside the USA: 001 200 µg/ml xylene cyanol FF (X4126) Sigma-Aldrich offers RNA sample buffers with (R1386) or without ethidium bromide (R4268) SYBR ® Green Nucleic Acid Stain (S9430) or ethidium bromide: 0. Methyl orange-xylene cyanol | C39H41N5Na2O10S3 | CID 90474202 - structure, chemical names, physical and chemical properties, classification, patents, literature Xylene cyanol FF Revision Date 26-Dec-2021 Irritation No information available Sensitization No information available Carcinogenicity The table below indicates whether each agency has listed any ingredient as a carcinogen. In a 1% gel it runs at around 500bp but the higher % of the gel the lower it will run. Despite its larger molecular weight of 670g/mol it runs faster than Xylene cyanol (539g/mol) because of its larger negative charge (hydroxyl groups). Add 3 µL of 1% xylene cyanol and bromophenol blue to the reactions. The rate of migration varies with gel composition. Address: 2145 Xylene Cyanol. X0015. ₹6,600. For Research Use Only. Supplied in one 1. 5 M EDTA 0. 4 mL bottle. 26. 00. 9 mL glycerol. This item has been discontinued and is no longer available. Xylene cyanol or tracking dye on a 6% Contains 2 dyes; bromophenol blue and xylene cyanol FF to track DNA migration during electrophoresis. E-Gel Sample Loading Buffer is supplied as a ready-to-use 1X solution, and is formulated specifically for maximum performance on E-Gel EX gels as well as other E-Gel precast gels. Procedure Gel preparation SIAL Xylene Cyanol FF. Description. CAS Common Chemistry. : 2650-17-1 108 of 108. Storage instructions. 4 mL tube. 7. Taken from CPMB UNIT 2. 00 Specifications Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Molecular formula. Jul 19, 2007 · Research. 4. 1. 05% xylene cyanol FF, 20 mM ethylenediaminetetraacetic acid (EDTA), pH 8. Xylene Cyanol FF OmniPur® Grade; CAS Number: 2650-17-1; Synonyms: OmniPur® Xylene Cyanol FF; find Millipore-9710OP MSDS, related peer-reviewed papers, technical documents Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. These are the same solutions we use in-house and in our On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. Published: 25 November 2002. Store under desiccating conditions. Xylene. Put wet filter paper on the vacuum porous stage and make sure the filter paper is in the area where the cut window of the green plastic gasket is going to be. Each dye has its own advantages and limitations and depending on that it is used in the Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl 2, KCl, tracking dyes and stabilizers. MDL Number. Colour Index. Ficoll 400 is a high molecular weight, neutral, hydrophilic, polysaccharide. MFCD00040657. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. : BP565-10 CAS No 2650-17-1 Synonyms Acid blue 147 Recommended Use Laboratory chemicals. These are the same solutions we use in-house and in our kits. Molecular Weight. Methyl Orange - Xylene Cyanol FF. Bromophenol blue, Xylene Cyanol, Bromocresol Green, Orange G and Cresol Red are common and popular dyes used in electrophoresis. 07) Component Compounds. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. Migration of xylene cyanol (bases) 30 2-8 6 20 20 8-25 8 28 10 25-35 12 55 8 35-45 19 75 6 45-70 26 105 5 70-300 35 130 4 100-500 ∼50 ∼230 aData, from Maniatis et al. No. 12. Ready-to-use electrophoresis buffers, gels, and powder stains Methyl Orange-xylene cyanol solution in water Revision Date 31-Jan-2023 Symptoms / effects,both acute and delayed No information available Endocrine Disruptor InformationNo information available Other Adverse Effects The toxicological properties have not been fully investigated. The migration of each dye was recorded after electrophoresis at 60 V for 60 min in a minigel. Fill the wells of the RNA gel with melted agarose. Details of the supplier of the safety data sheet Emergency Telephone Number CHEMTRECÒ, Inside the USA: 800-424-9300 CHEMTRECÒ, Outside the USA: 001-703 Xylene and bromophenol blue are the most commonly used dyes. If you are monitoring the progress of longer electrophoresis runs, Xylene Cyanol FF is the tracking dye of choice. *Items available in Japan warehouse will be dispatched in 10-12 working days. Bromophenol blue migrates with the 300bp fragment while xylene cyanol FF migrates with the 4000bp fragment. Development. *TCI frequently reviews storage conditions to optimize them. Details of the supplier of the safety data sheet Mar 3, 2023 · Alternatively, the bromophenol blue and xylene cyanol FF can be substituted with 0. Make sure urea is in solution before using. Jun 3, 2009 · Xylene cyanol (XC) is most commonly used as an agarose gel electrophoresis size marker. RUO – Research Use Only. Production. Store at room temperature, keep tightly sealed, and protect Methyl Orange Indicator Solution, Modified (Methyl Orange-Xylene Cyanol Mixed Indicator), Ricca Chemical $101. 5% EDTA, 0. Both tracking dyes, Bromophenol blue and Xylene cyanol FF, are soluble in water (solubility in water is ~ 1 mg/ml) and carry net negative charge at neutral or slightly basic pH (the pH of the electrophoresis Xylene Cyanol FF. Xylene cyanol migrates at the a rate equivalent to approximately 2,000 base pairs long. Both tracking dyes , Bromophenol blue and Xylene cyanol FF, are soluble in water (solubility in water is ~ 1 mg/ml) and carry net negative charge at neutral or slightly basic pH (the pH of the electrophoresis Sep 5, 2007 · These were xylene cyanol FF, methyl orange, cresol red, bromophenol blue, orange G, and ponceau S. 866. 0) and 1mM EDTA. Azul de bromofenol e alaranjado G podem também ser usados para este propósito. Personal Protective Equipment Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910. 1. Quiz yourself with questions and answers for bio lab 2 test, so you can be ready for test day. *INR price is exclusive of domestic taxes applicable. Synonym(s): Acid blue 147, Cyanol FF, XC, Xylene Cyanole FF. Linear Formula: C 25 H 27 N 2 NaO 6 S 2. Continue rinsing. PubChem Substance ID. 5 × TBE for 5 min. 25G. Jul 8, 2021 · Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. 43535 Recommended Use Laboratory chemicals. May 5, 2022 · We observed that an electrophoresis time of 25 minutes using program “EX1–2%” and 32 minutes using program “SizeSelect2%” produced equivalent migration of xylene cyanol on the gel (Figure 3D, white area in last two lanes equivalent to 1000nt). The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Th OSHA Vacated PELs: Xylene cyanol FF: No OSHA Vacated PELs are listed for this chemical. Shop Xylene Cyanol FF 85%, Thermo Scientific Chemicals at Fishersci. 25% agarose gel for RNA (P6222) in 8-well format. Dye content 75 %. The compound has also been used in a mobility shift analysis of ssDNA. Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Before the samples can be loaded on the gel, samples must be heat denatured by heating the samples between 70-90 °C for a few minutes. Toeprint gel loading buffer (2×): 0. In organic chemistry, xylene or xylol (from Greek ξύλον (xylon) 'wood'; [1] [2] IUPAC name: dimethylbenzene) are any of three organic compounds with the formula (CH3)2C6H4. 5: 100-1000: 460: 100: 5: 100-500: 260: 65: 8: 60-400: 160 Xylene cyanol FF, ~75% 10GR Product code 44070 Thermo Scientific . 25% bromophenol blue) to the RNA samples to a final concentration of 1X. 61. [ citation needed ] Generally speaking, Orange G migrates faster than bromophenol blue, which migrates faster than xylene cyanol, but the apparent "sizes" of these dyes (compared to DNA molecules) varies with the concentration of agarose and the buffer system used. Nov 25, 2002 · Spectrophotometric Determination of Cerium with Leuco Xylene Cyanol FF. The tracking dye typically migrates with the DNA molecules around 5kb. In addition, two highly sensitive spectrophotometric assays Product Name Xylene cyanol FF Cat No. Jun 3, 2009 · From OpenWetWare. 5 hours. Reconstitution. Xylene cyanol Molecular Formula C 25 H 28 N 2 O 7 S 2. After electrophoresis, soak the gel in 0. Ecological information Ecotoxicity Do not empty into drains. 12. 2. In addition, two highly sensitive spectrophotometric assays Jul 26, 2007 · 10 × Dye + glycerol stock solution 10 mM Tris, 1 mM EDTA, 50% vol/vol glycerol, 0. Empirical Formula (Hill Notation): C 25 H 27 N 2 NaO 6 S 2. Ethidium bromide staining. Explore quizzes and practice tests created by teachers and students or create one from your course material. A colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Formula Weight: 538. Notes. 1 (PubChem release 2021. Circular RNA migration shifted from 2000nt in the high voltage “EX1–2%” (upper gel) to Reconstitution. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis. Mix by pipetting up and down. Cite this Page Xylene cyanol. Kiran Kumar. P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. All Ambion™ Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. Despite its smaller molecular weight of 539g/mol it runs slower than bromophenol blue (670g/mol) because of its charge (1 net negative charge). Xylene cyanole FF | C25H27N2NaO6S2 | CID 23687514 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological Xylene cyanol/bromophenol blue (1%, w/v) (optional) 500 μL: 0. 001% wt/vol bromophenol blue, 0. Xylene cyanol FF (C 25 H 27 N 2 NaO 6 S 2; Molar mass – 538. Handling. 5 µg/mL prepared in distilled water; Transilluminator; Precast Agarose Gels. com. Add 5 mL water to the vial to dissolve. 1% bromphenol blue. DownloadManual. Synonym (s): Acid blue 147; Cyanol FF; XC; Xylene Cyanole FF. Revanasiddappa &. (1975), are for single-stranded DNA; RNA will migrate slightly more slowly than DNA of the same sequence and length. Feb 7, 2018 · The theoretical emission oscillator strength of xylene isomers was found to be in line with experimental fluorescence intensity findings, in the order of p-xylene > o-xylene > m-xylene in water. Xylene Cyanol FF is used as a tracking dye to monitor the progress of electrophoresis separations. Remove contact lenses, if present and easy to do. Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Oct 29, 2009 · The loading mix usually contains 90% formamide, 0. 3. 17% Bromophenol Blue. Molecular weight. Download PDF. 87577890. Uses advised against Food, drug, pesticide or biocidal product use. This difference in properties is derived from the position of the substituent as the methyl groups of the xylene molecule are substituted at ortho This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Heat at 50°C until dissolved. It is added to provide high density to the sample. Since DNA migration can not be seen during electrophoresis, these tracking dyes help to monitor the progress of agarose gel electrophoresis. When the gel has run far enough (the bromophenol blue should still be in the gel, especially if you are interested in bands that are less than 300 bp), view the gel using the imager and the UV box in the dark room. Bromophenol blue migrates through the gel at the same rate as a DNA fragment approximately 300 base pairs long. 42135 Acid blue 147, Cyanol FF, XC, Xylene Cyanole FF Apr 1, 2021 · Load the samples on a 12% denaturing PAA gel and run the gel (16. If the bands have clearly separated and you can determine the sizes, Documents. The faster-moving, purplish band is the dye bromophenol blue, the slower-moving, aqua band is xylene cyanol . 25% xylene cyanol, 0. 025% SDS, Xylene Cyanol, and Bromophenol Blue. BPB will run off at ~1. 1% xylene cyanol and 0. C. 5 – 1. 7% Pronunciation of xylene cyanol with 1 audio pronunciation and more for xylene cyanol. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR product onto agarose gels. The compound has been used in both electrophoresis polyacrylamide and agarose gels for various evaluations including DNA sequencing. 17% Xylene Cyanol, and 0. 3. The presence of glycerol ensures that the DNA in the ladder and sample The percentage of agarose gel influences the moving position of orange G and xylene cyanol FF in the gel. 5. 1200. The orange G and xylene cyanol FF co-migrates with ~50 bp and ~4000 bp DNA fragments in 1% agarose gel respectively. 42135. 2650-17-1. CAS, a division of the O xileno cianol pode ser usado como um marcador para monitorar o processo de eletroforese em gel de agarose [ 1] e eletroforese em gel de poliacrilamida; em géis de agarose a 1%, ele migra de maneira típica na mesma taxa de um fragmento de par de DNA de 4000 bases. The presence of glycerol ensures that the DNA in the ladder and sample forms a Bromophenol blue and Xylene cyanol are the two most commonly used tracking dyes for the analysis of DNA on agarose gel electrophoresis. 2% (w/v) Orange G, which migrates with the buffer front in a 15% polyacrylamide gel. NFPA HEALTH HAZARD SUPPLIER Company: Santa Cruz Biotechnology, Inc. of 300-500 bp and the xylene cyanol (light blue) at 2-4 kb. Xylene Cyanol FF sc-206012 Hazard Alert Code Key: EXTREME HIGH MODERATE LOW Section 1 - CHEMICAL PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME Xylene Cyanol FF STATEMENT OF HAZARDOUS NATURE CONSIDERED A HAZARDOUS SUBSTANCE ACCORDING TO OSHA 29 CFR 1910. Ordering Information. 500 μL 10% (w/v) SDS 200 μL 0. Computed by PubChem 2. *Upon orders receipt, Hyderabad stocks will be dispatched on the same day. Recommendations. 70 - $251. 025 g xylene cyanol O xileno cianol pode ser usado como um marcador para monitorar o processo de eletroforese em gel de agarose [ 1] e eletroforese em gel de poliacrilamida; em géis de agarose a 1%, ele migra de maneira típica na mesma taxa de um fragmento de par de DNA de 4000 bases. 在1%的凝胶中，二甲苯蓝以4000个DNA 碱基对的速率迁移。 共轭 Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others. キシレンシアノール（Xylene cyanol）は、アガロースゲル電気泳動とポリアクリルアミドゲル電気泳動の観測に使われるカラー Step 1: To prepare 10 ml of 6X DNA loading dye, weigh out 25 mg xylene cyanol FF and 4 g sucrose. Due to this, Xylene cyanol FF-containing single loading dyes are not safe to monitor DNA fragments of small sizes and you may risk losing them as they can run out of the gel. Thermo Scientific 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. An aliquot of intact RNA should always be run as a positive control to rule out unusual results due to gel artifacts. Wet the nylon membrane and filter paper first in H 2 O and then 10× SSC buffer. 8S and 5S (~150 nt): run 12% gel for 3. MDL Number: MFCD00040657. 538. 5% bromphenol blue and 0. Bromophenol blue (BPB) is most commonly used as an agarose gel electrophoresis size marker. 05. 05% bromophenol blue, 0. 5 cm wide, 24 cm long, 1 mm thick) in 1 × TBE at 20 to 25 mA. 001% wt/vol xylene cyanol FF. Empirical Formula: C 25 H 27 N 2 NaO 6 S 2. Run the gel in 1× TBE. 4. Dyes were run individually and as a mix of all six dyes together on gel-electrolyte combinations identified as suitable in Table IV . 4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA. Methyl orange-xylene cyanole FF solution; find Sigma-Aldrich-404001 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Download scientific diagram | Approximate gel mobilities of Bromophenyl Blue (BB) and Xylene Cyanol (XC) gel 501 loading dyes in polyacrylamide denaturing gels: 502 from publication: Fluorescent We would like to show you a description here but the site won’t allow us. 10'000 bp at 0. It is a pre-mixed loading buffer containing two different dyes xylene cyanol FF for visual tracking of DNA migration during agarose gel electrophoresis. The tracking dye typically migrates with the DNA molecules Xylene Cyanol FF, with the CAS number 2650-17-1, is a synthetic dye commonly used in molecular biology and biochemistry research, particularly in the visualization of biomolecules during electrophoretic techniques. 2 Strains used The N. Documents. 0 g/mol. 61 gram/mole) is available commercially as a dark green crystalline powder. Dissolve the content by inverting the tube number of times or using a rotator/vortexer until all the ingredients are dissolved completely. Storage 10mM Tris-HCl (pH 8. I. 일반 설명 Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. 05% (w/v) Add enough 10× TBE electrophoresis buffer (diluted to 1×) to reach the 10-mL mark in a 15-mL conical tube containing urea. : AC440700000, AC440700100, AC440700500 CAS No 2650-17-1 Synonyms Acid blue 147 Recommended Use Laboratory chemicals. Volume 18 , pages 1275–1278, ( 2002 ) Cite this article. crassa strain is the standard laboratory wild‐type designated Oak Ridge 74‐OR23‐IVA (Fungal Genetics Stock Center strain #2489). Alternatively, the dye can be added to a free lane on the gel to monitor electrophoresis and prevent possible interference with protein binding. C39H41N5Na2O9S3. 5-4 hours. Synonyms. Aug 30, 2018 · A loading dye, also known as tracking dye or sample loading dye is a dye-based solution added to the sample, prior to load. Na Discover more in SciFinder n. Hosakere D. On 0. #: XB0005 CAS Number: 2650-17-1 Grade: Indicator CTHH: C 25 H 27 N 2 NaO 6 S 2 Mô Tả: Dạng bột màu xanh lá cây hoặc xanh á cây đậm - Độ hòa tan: dd trong màu xanh đậm - Độ thất thoát sau khi sấy khô (ở 105 độ C): ≤10% - Thích hợp P302+P352-IF ON SKIN: Wash with plenty of water/. qs ha rl ur jx vd zl ve il cf